‘Click’ chemistry modifications
Click chemistry is a versatile technique for the synthesis of a variety of biomolecules conjugates such as Nucleic Acids (DNA/RNA), peptides, proteins, and antibodies. Besides of oligonucleotide labeling, click chemistry allows the multiple labeling of polymerized DNA strands via incorporation of Alkyne-dNTPs (triphosphates) followed by post-synthetic click reactions with high efficacy.
Most popular applications:
- Fluorescence In Situ Hybridization (FISH)
- qPCR-Assays
- DNA/RNA Microarrays
- Bioconjugation to Nanoparticles
- Fluorescence Microscopy
- Electrophoretic Mobility Shift Assay (EMSA)
- Fluorescence Activated Cell Sorting (FACS)
- Flow Cytometry (FCM), Cell Tracking and Cell-based Assays
- Fluorescence Correlation Spectroscopy (FCS)
Azides
Функциональная группа терминальный азид
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