Catalog

LNA (Locked Nucleic Acid) RNA modification

Locked nucleic acids have restricted conformation of the sugar moiety formed by methylene bridge between oxygen atom at 2’-position and carbon at 4’-position. Such predefined conformation of ribose determines excellent hybridization of oligonucleotides even if they bear only 3-5 LNA residues. LNAs can be inserted into both RNA and DNA oligonucleotides, and their presence increases the Tm by 2 to 4° per LNA. LNAs are also widely applied because they enhance hybridization stability and biostability of oligonucleotides in vivo [https://doi.org/10.1016/B978-0-12-407676-1.00002-0]. LNA oligonucleotides are used in ASO, primers and probes for PCR, in ribozymes, FISH probes (fluorescent in situ hybridization), molecular beacons for SNP (single nucleotide polymorphism) identification, microarrays, etc.

Available sites for modifications are 5’, internal, and 3’.