Catalog

Molecular beacons

Molecular beacons are hairpin-shaped oligonucleotides with the internally quenched fluorophore. Molecular beacons are designed in the way to remain an intact stem-loop structure during primer elongation, while after each cycle they bind to amplicon to quantify the target. Also, you can order molecular beacons with additional chemical modifications to increase thermal stability and specificity.

Detection of single nucleotide polymorphisms (SNP)
Allele discrimination, gene copy number variation (CNV)
Multiplexing for mutation detection
Choose from a wide variety of reporter and quencher combinations for accurate analysis

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Modifications

Fluorophore-Quencher pairs for qPCR Dual-labeled Probes*

* For internal modifications - Quenchers and fluorophores are introduced via dT only. Contact us if you did not find the desired modification. Learn more about modifications here.
**If you are unable to find modifications of your interest, please contact us info@genterra.ru
Fluorophore	Quencher**
FAM	BHQ1
R6G
SIMA
HEX
R6G	BHQ2
VIC
JOE
HEX
TAMRA
ROX
Cy5
Cy5.5	BHQ3
Cy5.5

Molecular beacons form a stem-loop structure that bring a 5′ fluorescent dye and a 3′ quencher together. Stem can be universal for all probes, while the loop is able to hybridize to the target DNA with high specificity. As a result, the reporter dye and quencher are separated sufficiently to induce the increase of fluorescence. During amplification these probes only bind to DNA without degradation by Taq polymerase.