Fluorescent oligonucleotides are used in a variety of biochemical and analytical applications, such as DNA sequencing, FISH, qPCRand others. We manufacture a lot of different reagents for the synthesis of fluorescent oligonucleotides. Whether you need to make single, multi or dual labeled probes, we have the fluorophores and quenchers you need.
Fluorophores
For Internal modifications - Quenchers and Fluorophores are incorporated via dT.
Contact us, if you were unable to find modifications of your interest.
Fluorophores
5'
Int*
3'
Emission color
λex, nm
λem, nm
εex, l mol-1 cm-1
Quantum yield
FAM
✓
✓
✓
Green
494
520
75000
0.9
R6G
✓
✓
✓
Yellow-Green
518
542
116000
0.95
VIC (SIMA)
✓
✓
✓
Yellow-Green
531
555
92300
0.63
JOE
✓
Yellow-Green
533
554
75000
0.61
HEX
✓
✓
✓
Yellow
533
549
87770
0.57
TAMRA
✓
✓
✓
Yellow
541
567
84000
0.1
ROX
✓
✓
✓
Orange
570
591
93000
1.00
Cy5
✓
✓
✓
Red
646
662
271000
0.2
Cy5.5
✓
✓
✓
Red
684
710
198000
0.2
Extinguishers
*GTQs are full chemical analogues of BHQs ™ For Internal modifications - Quenchers and Fluorophores are incorporated via dT.
Contact us, if you were unable to find modifications of your interest.
Extinguishers
5'
Int*
3'
λmax, nm
Extinguishing range, nm
GTQ1**
✓
✓
✓
534
480-580
GTQ2**
✓
✓
✓
579
550-650
GTQ3**
✓
672
620-730
Recommended Fluorophore Quencher Pairs for Double Labeled qPCR Probes and Molecular Beacons*
*For Internal modifications - Quenchers and Fluorophores are incorporated via dT.
Contact us, if you were unable to find modifications of your interest.
