Catalog

2’-deoxy-Uridine (dU)

2’-deoxyuridine (dU) can be used instead of thymidine residues in DNA oligonucleotides. The most frequent mutation in the cell – deamination of dC in DNA results in dU formation. dU in DNA is recognized by DNA reparation enzymes - uracil-DNA-glycosylases which subsequently removes uracil followed by strand scission and reparation.

This system can be used to decrease lab contamination with PCR products. As dUTP is a good substrate for Taq DNA polymerase, so one can fully or partially substitute dTTP with dUTP. Addition of thermolabile uracil-DNA-glycosylase to the test tube allows eliminate DNA contamination, while heating during PCR lead to UDG denaturation without any interference with further amplification.