2'-MOE (2'-O-Methoxyethyl) RNA modification
2’-MOE oligonucleotides demonstrate enhanced binding affinity to the target RNAs, serum proteins and an excellent resistance to ubiquitous nucleases in vitro and in vivo [DOI: 10.1016/j.jbc.2021.100416]. 2’-MOE RNA modification is introduced in 5’-/3’- wings of gapmer ASO to increase RNA binding affinity and resistance to nucleases. Duplexes of fully 2'-MOE-modified oligonucleotides with RNA does not support RNase H activity, which makes them useful as splice-switching oligonucleotides.
Available sites for modifications are 5’, internal, and 3’.
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