Catalog

Custom Dual labeled qPCR probes

Dual-labeled probe is an oligonucleotide with a fluorescent dye and a quencher that can bind to the complementary DNA strand during amplification. Before the annealing step in PCR the probe is intact and fluorescence is quenched as a fluorophore and a quencher are in close proximity. DNA polymerase during elongation reaches the probe and cleaves the hybridized probe that leads to the increase of fluorescence as a fluorescent dye and a quencher are not connected anymore. Fluorescence increases in each cycle, proportional to the rate of probe cleavage. This type of probes allows to improve the accuracy and selectivity of RT-qPCR.

Select from a wide range of license-free dyes, including FAM, R6G, VIC, HEX, JOE, TAMRA, ROX and Cy® 5 and Cy5.5 dyes
Suitable for multiplex detection of several DNA targets in one tube
Achieve high efficiency of qPCR under fast or standard cycling conditions
Minimize troubleshooting: probes are HPLC-purified and verified by mass spectrometry*

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Mods

Fluorophore-Quencher pairs for qPCR Dual-labeled Probes*

*For Internal modifications - Quenchers and Fluorophores are incorporated via dT. To learn more about modifications follow this link.
If you are unable to find modifications of your interest, please contact us info@genterra.ru
Fluorophore	Quencher**
FAM	TAMRA BHQ1
R6G
SIMA
HEX
R6G	BHQ2
VIC
JOE
HEX
ROX
Cy5
Cy5.5
Cy5.5	BHQ3

Dual labeled probes used in quantitative PCR take an advantage of the 5'→3' exonuclease activity of Taq polymerase. Probes contain a fluorescent dye and the appropriate quencher covalently linked to the 5′ and 3′ end of the custom oligo, which anneals with the target DNA between the PCR primers. During the extension phase of PCR, the 5'→3' exonuclease activity of Taq polymerase cleaves the fluorescent probe and fluorescent signal increases and accumulates as the number of PCR cycles